Original Research Article
Year: 2020 | Month: September | Volume: 7 | Issue: 9 | Pages: 447-452
Utility of Modified PAP Stain in Histopathology for Demonstration of Keratin
1Shyama S, 2Ruby Elizabeth Elias, 3Bindiya Gisuthan
1Postgraduate, Dept. of MLT, Govt. Medical College, Thiruvananthapuram, Kerala.
2Associate Professor (CAP), Dept.of Pathology, Govt. Medical College, Thiruvananthapuram, Kerala.
3Associate Professor (CAP), Dept. of Pathology, Govt. Medical College, Thiruvananthapuram, Kerala.
Corresponding Author: Ruby Elizabeth Elias
ABSTRACT
Introduction: Keratins are intermediate filament proteins which give structural stability to the cell. In routine Hematoxylin & Eosin stain, they appear as eosinophilic material. A few other stains are described in literature for the demonstration of keratin. The aim of this study was to assess the utility of modified PAP stain in the demonstration of keratin in histopathology sections. Materials and Methods: In this study, 89 histopathological cases showing keratin were included. These were comprised of various benign and malignant lesions involving squamous epithelium. Two sections were taken from the paraffin block of each case. H &E stain and modified PAP stain were done in all. These two stains were compared based on 4 parameters: Staining quality, background clarity, morphology and differentiation. Scores were allotted ranging from 1 to 3 for each parameter. They were statistically analysed by ‘unpaired t test’ at 95% confidence level (p ≤ 0.05) Results: It was found that there was no significant difference in the staining characteristics between two stains, based on the 4 parameters. But individual lesions show variable staining of keratin when the two stains were compared. Discussion and Conclusion: A few available similar studies showed a distinct advantage of modified PAP compared to H&E. In our study modified PAP was found useful in only a few conditions like squamous cell carcinomas in which the differentiation or contrast was excellent when compared to H&E stain.
Keywords: Keratin, Modified PAP, Hematoxylin & Eosin
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