Original Research Article
Year: 2019 | Month: July | Volume: 6 | Issue: 7 | Pages: 121-126
Effect of Storage on the Stability of Enzyme Activities in Pooled Serum
Priyanka Prasad1, Rakesh Kumar2, Santosh Kumar3
1Assistant Professor, Dept of Biochemistry, NMCH, Patna;
2Associate Professor, Dept of Pediatrics, NMCH, Patna;
3Associate Professor & HOD, Dept of Biochemistry, NMCH, Patna.
Corresponding Author: Rakesh Kumar
ABSTRACT
Objective: To assess the stability of enzymatic parameters in home-made Frozen Human Serum on storage and compare it with commercially prepared lyophilized human sera already being used in our laboratory at IGIMS, Patna.
Methods: The home made QC serum was prepared from twenty healthy volunteers and was screened for HIV and HBV, pooled together and stabilized with 0.1%Sodium Azide. Preliminary control limits (i.e. Mean, SD ) was calculated from 20 runs of first month for three enzymatic parameters and results were compared with those of commercially available lyophilized human sera.
Results: ALP had narrower coefficients of variation in the home made serum making it a stable control material in comparison to the commercial ones whereas other enzymatic parameters showed unsteadiness over the 6 months storage period.
Conclusion: We conclude that the activity of ALP in pooled sera was stable when stored frozen at -200c over the 6 months storage period.
Key words: enzymatic parameters, lyophilized human sera, pooled, storage.
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