Original Research Article
Year: 2019 | Month: July | Volume: 6 | Issue: 7 | Pages: 1-10
Study of Cellular Morphometry in Colorectal Epithelial Lesions with Clinicopathological Correlation
Dr. Koushik Chakraborty1, Dr. Sucharita Sarkar2, Dr. Asim Kumar Manna3, Dr. Saswati Sengupta4, Dr. Mousumi Bag4
1Consultant Pathologist, Mohan’s Diabetic Clinic, Kolkata, West Bengal
2Demonstrator, R.G Kar Medical College & Hospital, Kolkata -700004, West Bengal.
3Professor, Institute of Post Graduate Medical Education and Research, Kolkata-700 020, West Bengal
4Post Graduate Trainee, Dept of Pathology, Institute of Post Graduate Medical Education and Research, Kolkata-700 020, West Bengal
Corresponding Author: Dr. Sucharita Sarkar
ABSTRACT
Diseases of the colon and rectum v.i.z. different types of colitis, polyps and colorectal carcinoma are representing a wide spectrum of differential diagnosis. This study was expected to classify different colorectal epithelial lesion with the help of various cellular morphometry in different types of lesions. In the study, morphometric parameters including nuclear and cytoplasmic dimensions as well as nucleo/cytoplasm ratio in different colonic and rectal epithelial lesions. Juvenile Polyp v/s Adenomatous Polyp showed significant difference between mean nuclear diameter(p value=0.0013), mean nuclear area(p value=0.0015) and mean nuclear perimeter(p value=0.0013) & nucleo-cytoplasmic ratio (p value=0.00003) Polyp. Adenomatous Polyp v/s Adeno Carcinoma showed the mean cytoplasmic diameters(p value=0.00002) and the nucleo-cytoplasmic ratio(p value=0.00003) did show immensely significant difference and so Adeno Carcinoma v/s Poorly- differentiated Carcinoma( mean nuclear diameter(p value=0.006), mean nuclear area(p value=0.0023) and mean nuclear perimeter (p value=0.006) nucleo-cytoplasmic ratio(p value=0.00180). So, it was concluded that even with the advent and popularity of immunohistochemistry, morphometric study was also found reliable resolving diagnostic dilemmas between benign, premalignant and malignant colorectal epithelial lesions.
Key words: Colorectal carcinoma, Polyp, Morphometry, Nuclear diameter
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