Original Research Article
Year: 2019 | Month: December | Volume: 6 | Issue: 12 | Pages: 33-40
Oxidative Stress in Alcoholic Liver Disease
Jasvir Singh
Assistant Professor, Department of Biochemistry, Govt. Medical College & Rajindra Hospital Patiala.
ABSTRACT
Background: Alcoholic Liver disease is mainly of three types:-
Alcoholic fatly liver; Alcoholic hepatitis; and alcoholic cirrhosis. During the metabolism of alcohol in the liver, produces highly reactive molecules that can destroy vital cell components through a chemical process called oxidation. Oxidative stress increases the rate of production of free radicals hence induces lipid peroxidation.
Antioxidants are natural defence mechanism. Existing in our system and these are capable of scavenging the deleterious free radicals.
Aims: To study the activity of serum GGT Serum, MDA and GSH in whole blood in the patients with alcoholic liver disease and its comparison with controls and to study the correlation of serum MDA with serum GGT and blood GSH in the patients with alcoholic liver disease.
Material And Method: The study was conducted on 50 diagnosed cases of alcoholic liver disease visiting Rajindra Hospital, Patiala and 50 healthy subjects of matched age & sex served as controls and Biochemical Investigations of Serum GGT, Serum MDA and GSH in whole blood were conducted in the department of Biochemistry Govt. Medical College Patiala and the results were statistically analysed.
Results: The mean values of serum GGT in study group and control group were 20.80±6.38 and 234.44±204.31(IU/L) respectively (Normal value of GGT 0-50 (IU/L), mean values of serum MDA in study group and control group were 22.00±5.03 and 53.90±11.43(µmol/L) respectively and mean values of blood glutathione in control group and study group were 41.6±4.51 and 18.40±2.39(mg%) respectively the decrease mean blood Glutathione level in study group as compared to mean blood Glutathione level in control group shows statistically significant association (p value < 0.001).
Conclusion: Our study shows that there was increased levels of Serum MDA, Serum GGT and decreased levels of blood glutathione in the study group and there was a positive correlation between Serum MDA levels and Serum GGT levels in the study group and there was a negative correlation between serum MDA levels blood glutathione levels in the study group. This correlation predicts that during increased oxidative stress, the GSH was utilized to counter the oxidative stress due to alcoholic liver disease.
Key words: GGT (gamma glutamyl transferase) ALD (Alcoholic liver disease), GSH (reduced glutathione), LPO (Lipid Peroxidation) MDA (Malonyldialdehyde), ROS(Reactive oxygen species), SOD (superoxide dismutase).
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