Year: 2025 | Month: February | Volume: 12 | Issue: 2 | Pages: 71-83
DOI: https://doi.org/10.52403/ijrr.20250209
A Comprehensive Review on HPTLC Method Development
Shital Narwade1, Dr. Hemalata Wadkar2, Aishwarya Ghanawat3, Krutika Nilwani4
Abhinav Education Society’s College of Pharmacy (B.Pharm), Narhe ,Pune-411041
Corresponding Author: Dr. Hemalata Wadkar
ABSTRACT
High-Performance Thin-Layer Chromatography (HPTLC) is a widely utilized analytical technique that offers significant advantages in the qualitative and quantitative analysis of a variety of chemical substances. This review aims to provide a comprehensive overview of HPTLC method development, focusing on its principles, instrumentation, key parameters, and applications. The paper discusses the critical steps involved in HPTLC method development, including selection of stationary phases, mobile phases, detection methods, and optimization of experimental conditions to achieve high resolution, sensitivity, and reproducibility. The review also highlights the importance of sample preparation, validation procedures, and the integration of HPTLC with other analytical techniques, such as mass spectrometry (MS) and spectrophotometry, to enhance its capabilities. Furthermore, the article explores the wide range of applications of HPTLC in pharmaceutical, environmental, food, and forensic analysis. This review aims to serve as a valuable resource for researchers and analytical chemists involved in the development and optimization of HPTLC methods for complex matrix analysis.
Keywords: HPTLC, Method development, Validation, Instrumentation, Qualitative analysis, Chromatography
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