Original Research Article
Year: 2023 | Month: January | Volume: 10 | Issue: 1 | Pages: 1-10
DOI: https://doi.org/10.52403/ijrr.20230101
Evaluation of Hexokinase 2 and Serum LDH in B-cell Non-Hodgkin’s Lymphoma Patients
Dr. Kruti Rajvik1, Pooja Parmar2, Dr. Beena Bhrambahtt3, Dr. Hemangini Vora4
1Junior Scientific Officer Immunohematology Lab, The Gujarat Cancer & Research Institute Medicity, Asarwa, Ahmedabad-380016
2M. Sc Cancer-Biology Student, Immunohematology Lab, The Gujarat Cancer & Research Institute Medicity, Asarwa, Ahmedabad-380016
3Asssitant Professor, Oncopathology Department, The Gujarat Cancer & Research Institute Medicity, Asarwa, Ahmedabad-380016
4Professor and Head, Cancer Biology Department, Head Immunohematology Lab, The Gujarat Cancer & Research Institute Medicity, Asarwa, Ahmedabad-380016
Corresponding Author: Dr Hemangini Vora
ABSTRACT
Background and Objective: Tumor cells produce energy by glycolysis and Lactate Dehydrogenase and Hexokinase are the key part of glycolysis, The present study aimed to evaluate the expression of Hexokinase 2 and serum LDH in B-cell Non-Hodgkin’s Lymphoma patients
Method: A total of 84 B-cell Non-Hodgkin’s Lymphoma Patients were enrolled in the study. HK-2 Marker was studied by Immunohistochemistry and LDH analysis was done by Cobas 6000 analyser method and correlated with Clinico-Pathological Parameters and Diagnostic panel.
RESULT:73% of B-cell NHL patients had Abnormal LDH. It was significantly correlated with Multiple Disease site (P=0.002). In correlation with Diagnostic Panel (MIb1, CD10, CD2, MUM1, BCL6, BCL2) a higher trend of Abnormal LDH was seen in BCL-6 positive and BCL-2 negative Patients. 64% of patients had HK-2 expression. In correlation with Clinical parameters, it was significantly correlated with >50 age group, while in correlation with pathological parameters A significant correlation observed with single disease site as compared to multiple disease site (P=0.004), In correlation with Diagnostic panel A higher trend of HK-2 Expression was observed in CD10 Negative and BCL-2 Negative Patients. Further Univariate survival analysis of LDH and HK-2 showed similar Overall survival in Abnormal and normal LDH level patients and HK-2 Positive and negative Patients respectively. Further Intercorrelation of both markers, showed almost similar expression of HK-2 between patients with normal LDH level and abnormal LDH level.
CONCLUSION: Abnormal LDH level was closely associated with patients having disease at multiple sites, suggesting its activity to be associated with the advancement of the disease, HK-2 positivity was related with older age group, strengthens the findings of increased altered metabolic activity with older age, They are also associated with BCL-2 negativity that indicates that anti-apoptotic activity of BCL-2 might be responsible for that Though there was no significant correlation of LDH,, HK-2 with overall survival, majority of the patients who were dead had abnormal LDH and half of them showed positive HK-2 expression
Briefly our data indicates that LDH and HK-2 can be useful parameter in defining clinical and prognostic aspects in NHL patient but needs further evaluation with larger groups of patients
Keywords: NHL, Altered metabolism, Hexokinase, LDH
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